验证数据展示
经过测试的应用
| Positive IP detected in | THP-1 cells |
| Positive IHC detected in | human cerebellum tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25949-1-AP targets CSF1R/CD115 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag22845 Product name: Recombinant human CSF1R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-369 aa of BC047521 Sequence: IPVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSILSTNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFEDQDALLPCLLTDPVLEAGVSLVRVRGRPLMRHTNYSFSPWHGFTIHRAKFIQSQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEAAQIVCSASSVDVNFDVFLQHNNTKLAIHQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANVTTKDTYRHTFTLSLP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | colony stimulating factor 1 receptor |
| 别名 | CSF1R, C FMS, CD115, CSF 1 R, CSF-1 receptor |
| 计算分子量 | 972 aa, 108 kDa |
| GenBank蛋白编号 | BC047521 |
| 基因名称 | CSF1R/CD115 |
| Gene ID (NCBI) | 1436 |
| RRID | AB_2880306 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07333 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CSF1R/CD115 antibody 25949-1-AP | Download protocol |
| IHC protocol for CSF1R/CD115 antibody 25949-1-AP | Download protocol |
| IP protocol for CSF1R/CD115 antibody 25949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Excessive MYC Orchestrates Macrophages induced Chromatin Remodeling to Sustain Micropapillary-Patterned Malignancy in Lung Adenocarcinoma | ||
Cell Rep Med Tumor heterogeneity and tumor-microglia interactions in primary and recurrent IDH1-mutant gliomas | ||
J Immunother Cancer Antagonism of estrogen-related receptor-α inhibits mitochondrial oxidative phosphorylation and reduces M2 macrophage infiltration in endometrial cancer | ||
J Transl Med ITIH5-mediated fibroblast/macrophage crosstalk exacerbates cardiac remodelling after myocardial infarction | ||
Front Immunol Identification of key genes in membranous nephropathy and non-alcoholic fatty liver disease by bioinformatics and machine learning. | ||
CNS Neurosci Ther A novel chemokine-based signature for prediction of prognosis and therapeutic response in glioma |
