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验证数据展示

  • WB analysis of KYSE-30 using 27785-1-AP (same clone as 27785-1-PBS)

    WB analysis of KYSE-30 using 27785-1-AP (same clone as 27785-1-PBS)

    WB result of CSDA antibody (27785-1-AP; 1:1000; room temperature for 1.5 hours) with wild-type and CSDA knockout KYSE-30 cells. This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

  • WB analysis using 27785-1-AP (same clone as 27785-1-PBS)

    WB analysis using 27785-1-AP (same clone as 27785-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27785-1-AP (CSDA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 27785-1-AP (same clone as 27785-1-PBS)

    IHC staining of mouse heart using 27785-1-AP (same clone as 27785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 27785-1-AP (same clone as 27785-1-PBS)

    IHC staining of mouse skeletal muscle using 27785-1-AP (same clone as 27785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27785-1-AP (CSDA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 27785-1-AP (same clone as 27785-1-PBS)

    IF Staining of A431 using 27785-1-AP (same clone as 27785-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CSDA antibody (27785-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27785-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CSDA Polyclonal antibody, PBS Only

CSDA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27785-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

cold shock domain protein A, Cold shock domain-containing protein A, DBPA, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27785-1-PBS targets CSDA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26839

Product name: Recombinant human CSDA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC008801

Sequence: MSEAGEATTTTTTTLPQAPTEAAAAAPQDPAPKSPVGSGAPQAAAPAPAAHVAGNPGGDAAPAATGTAAAASLAAAAGSEDAEKKVLATKVLGTVKWFNVRNGYGFINRNDTKEDVFVHQTAIKKNNPRKYLRSVGDGETVEFDVVEGEKGAEAANVTGPDGVPVEGSRYAADRRRYRRGYYGRRRGPPRNYAGEEEEEGSGSSEGFDPPATDRQFSGARNQLRRPQYRPQYRQRRFPPYHVGQTFDRRSRVLPHPNRIQAGEIGEMKDGVPEGAQLQGPVHRNPTYRPRYRSRGPPRPR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cold shock domain protein A
别名 cold shock domain protein A, Cold shock domain-containing protein A, DBPA, DNA-binding protein A, Single-strand DNA-binding protein NF-GMB
计算分子量 40 kDa
观测分子量60-68 kDa
GenBank蛋白编号BC008801
基因名称 CSDA
Gene ID (NCBI) 8531
RRIDAB_3085996
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16989
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CSDA (Cold shock domain protein A), also known as DBPA (DNA-binding protein A), YBX3 (Y-box protein 3), is a DNA-binding protein that represses angiogenesis and lymphangiogenesis by directly binding to hypoxia response element (HRE) and serum response element (SRE). CSDA is ubiquitous expressed in human skeletal muscle, heart, and decidual cells, which is thought to a multifunctional epithelial-specific protein involved in gene transcription, DNA repair, and interaction with other proteins (PMID: 33816248). CSDA is a nucleic-acid-binding protein that directly regulates gene expression by using different mechanisms, and acts as a translational repressor (e.g., MYH9 and SPTBN1) and positive regulator of mRNA stability (PMID: 31189097). Western blot analysis detected CDSA at a range of 50~70 kDa due to the anomalous mobility of Y-box proteins (PMID: 38255791).

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