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Citrate synthase Polyclonal antibody

Citrate synthase Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

IF/ICC, IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16131-1-AP

Print datasheet

Synonyms

Citrate synthase, CS



经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CS Ready-To-Use IHC Kit. CS primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16131-1-AP targets Citrate synthase in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, rabbit, hamster
Immunogen Citrate synthase fusion protein Ag9117 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name citrate synthase
Synonyms Citrate synthase, CS
Calculated Molecular Weight 466 aa, 52 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC010106
Gene Symbol CS
Gene ID (NCBI) 1431
RRIDAB_1640013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75390
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

实验方案

Product Specific Protocols
WB protocol for Citrate synthase antibody 16131-1-APDownload protocol
IHC protocol for Citrate synthase antibody 16131-1-APDownload protocol
IF protocol for Citrate synthase antibody 16131-1-APDownload protocol
IP protocol for Citrate synthase antibody 16131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.

Authors - Miranda Y Fong
mouseIF

Nat Neurosci

A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto
humanIF

ACS Cent Sci

A Selective SARS-CoV-2 Host-Directed Antiviral Targeting Stress Response to Reactive Oxygen Species

Authors - Cong Tang
humanIF

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
humanWB

Nat Commun

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Authors - Xiaoyu Ma
mouseWB,IF

Aging Dis

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging

Authors - Minghao Yuan