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验证数据展示

  • WB analysis of COS-7 using 16894-1-AP

    WB analysis of COS-7 using 16894-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 16894-1-AP (CRYZL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 16894-1-AP

    IHC staining of human ovary tumor using 16894-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16894-1-AP (CRYZL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 16894-1-AP

    IHC staining of human ovary tumor using 16894-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16894-1-AP (CRYZL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CRYZL1 Polyclonal antibody

CRYZL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16894-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, ELISA

别名

4P11, CRYZL1, Protein 4P11, QOH 1, Zeta crystallin homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOS-7 cells, MCF-7 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16894-1-AP targets CRYZL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原

CatNo: Ag10419

Product name: Recombinant human CRYZL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-113 aa of BC013155

Sequence: MKGLYFQQSSTDEEITFVFQEKEDLPVTEDNFVKLQVKACALSQINTKLLAEMKMKKDLFPVGREIAGIVLDVGSKVSFFQPDDEVVEVLSRLGAVAHVCNPSTLGGQGGRIT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 crystallin, zeta (quinone reductase)-like 1
别名 4P11, CRYZL1, Protein 4P11, QOH 1, Zeta crystallin homolog
计算分子量 349 aa, 39 kDa
观测分子量 39-45 kDa
GenBank蛋白编号BC013155
基因名称 CRYZL1
Gene ID (NCBI) 9946
RRIDAB_2878329
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95825
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CRYZL1 antibody 16894-1-APDownload protocol
IHC protocol for CRYZL1 antibody 16894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols