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验证数据展示

  • WB analysis of COS-7 using 16894-1-AP

    WB analysis of COS-7 using 16894-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 16894-1-AP (CRYZL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 16894-1-AP

    IHC staining of human ovary tumor using 16894-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16894-1-AP (CRYZL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 16894-1-AP

    IHC staining of human ovary tumor using 16894-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16894-1-AP (CRYZL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CRYZL1 Polyclonal antibody

CRYZL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16894-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, ELISA

别名

4P11, CRYZL1, Protein 4P11, QOH 1, Zeta crystallin homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOS-7 cells, MCF-7 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16894-1-AP targets CRYZL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原 CRYZL1 fusion protein Ag10419 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 crystallin, zeta (quinone reductase)-like 1
别名 4P11, CRYZL1, Protein 4P11, QOH 1, Zeta crystallin homolog
计算分子量 349 aa, 39 kDa
观测分子量 39-45 kDa
GenBank蛋白编号BC013155
基因名称 CRYZL1
Gene ID (NCBI) 9946
RRIDAB_2878329
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95825
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CRYZL1 antibody 16894-1-APDownload protocol
IHC protocol for CRYZL1 antibody 16894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols