验证数据展示
经过测试的应用
| Positive WB detected in | rat eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16542-1-AP targets CRYGS in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | CRYGS fusion protein Ag9868 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | crystallin, gamma S |
| 别名 | Beta crystallin S, CRYG8, CRYGS, crystallin, gamma S, Gamma crystallin S, Gamma S crystallin, GRYG8 |
| 计算分子量 | 178 aa, 21 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC070241 |
| 基因名称 | CRYGS |
| Gene ID (NCBI) | 1427 |
| RRID | AB_3669249 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P22914 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRYGS (Gamma-S crystallin), also called CRYG8, belongs to the gamma-crystallins (PMID: 7733876; 23199295). Three major classes of crystallins, alpha, beta, and gamma, are common to the eye lens throughout all vertebrates. The beta and gamma classes are made up of homologous proteins and constitute the beta/gamma crystallin superfamily. CRYGS is expressed late but abundantly in the ocular lens (PMID: 1445197). It is also expressed outside the lens, particularly in the mature retina and cornea. In contrast to the beta-crystallins which associate in various combinations to form low or high molecular weight aggregates, CRYGS is, like the other gamma-crystallins, a monomeric protein (PMID: 16141006).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRYGS antibody 16542-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |