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CRYBB3 Polyclonal antibody

CRYBB3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21009-1-AP

Print datasheet

Synonyms

Beta B3 crystallin, Beta crystallin B3, CATCN2, CRYB3, CRYBB3, crystallin, beta B3

验证数据展示

  • WB analysis using 21009-1-AP

    WB analysis using 21009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21009-1-AP (CRYBB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse eye using 21009-1-AP

    IHC staining of mouse eye using 21009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21009-1-AP (CRYBB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse eye tissue, rat eye tisssue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 21009-1-AP is CRYBB3 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen CRYBB3 fusion protein Ag15212 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name crystallin, beta B3
Synonyms Beta B3 crystallin, Beta crystallin B3, CATCN2, CRYB3, CRYBB3, crystallin, beta B3
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC069479
Gene Symbol CRYBB3
Gene ID (NCBI) 1417
RRIDAB_10732686
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26998
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CRYBB3 antibody 21009-1-APDownload protocol
IHC protocol for CRYBB3 antibody 21009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Lab Invest

Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway.

Authors - Wenting Yu
View Article