验证数据展示
经过测试的应用
Positive WB detected in | mouse eye tissue, rat eye tisssue |
Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
21009-1-AP targets CRYBB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | CRYBB3 fusion protein Ag15212 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | crystallin, beta B3 |
别名 | Beta B3 crystallin, Beta crystallin B3, CATCN2, CRYB3, CRYBB3, crystallin, beta B3 |
计算分子量 | 211 aa, 24 kDa |
观测分子量 | 24 kDa |
GenBank蛋白编号 | BC069479 |
基因名称 | CRYBB3 |
Gene ID (NCBI) | 1417 |
RRID | AB_10732686 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P26998 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for CRYBB3 antibody 21009-1-AP | Download protocol |
IHC protocol for CRYBB3 antibody 21009-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |