验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, U2OS cells, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15808-1-AP targets Alpha B Crystallin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, fish |
| 免疫原 |
CatNo: Ag8543 Product name: Recombinant human aB-Crystallin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-175 aa of BC007008 Sequence: MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFPTSTSLSPFYLRPPSFLRAPSWFDTGLSEMRLEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPERTIPITREEKPAVTAAPKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | crystallin, alpha B |
| 别名 | CRYAB, Alpha crystallin B chain, Alpha(B) crystallin, Alpha(B)-crystallin, Alpha-crystallin B chain |
| 计算分子量 | 175 aa, 20 kDa |
| 观测分子量 | 20-22 kDa |
| GenBank蛋白编号 | BC007008 |
| 基因名称 | Alpha B Crystallin |
| Gene ID (NCBI) | 1410 |
| RRID | AB_2292175 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P02511 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alpha B-crystallin, encoded by CRYAB gene, is multifunctional, serving as both a major structural protein in the lens and a small heat-shock protein in other tissues in mammals. Alpha B-crystallin may contribute to the transparency and refractive index of the lens. Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. Mutations in the CRYAB gene cause distinct clinical phenotypes including isolated posterior polar cataract, myofibrillar myopathy, cardiomyopathy, or a multisystemic disorder combining all these features. Impairment of alpha-B crystallin dimerization may be relevant to the pathogenesis of these disorders.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Alpha B Crystallin antibody 15808-1-AP | Download protocol |
| IHC protocol for Alpha B Crystallin antibody 15808-1-AP | Download protocol |
| IP protocol for Alpha B Crystallin antibody 15808-1-AP | Download protocol |
| WB protocol for Alpha B Crystallin antibody 15808-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis MiRNA-671-5p Promotes prostate cancer development and metastasis by targeting NFIA/CRYAB axis. | ||
J Hazard Mater Maternal exposure to E 551 during pregnancy leads to genome-wide DNA methylation changes and metabolic disorders in the livers of pregnant mice and their fetuses | ||
Food Res Int Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella). | ||
Oxid Med Cell Longev Resveratrol Modulation of Protein Expression in parkin-Mutant Human Skin Fibroblasts: A Proteomic Approach. |
