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- KD/KO Validated
CRTC2,TORC2 Polyclonal antibody
CRTC2,TORC2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IF/ICC, IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse liver tissue, HEK-293 cells, mouse kidney, rat kidney |
| Positive IHC detected in | human skin cancer tissue, human gliomas tissue, rat kidney tissue, human urothelial carcinoma tissue, mouse lung tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
Planning an IHC experiment? We recommend our IHCeasy CRTC2 Ready-To-Use IHC Kit. CRTC2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12497-1-AP targets CRTC2,TORC2 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, bovine |
| Immunogen | CRTC2,TORC2 fusion protein Ag3167 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CREB regulated transcription coactivator 2 |
| Synonyms | CRTC2, CRTC2,TORC2, RP11 422P24.6, TORC 2, TORC2, Transducer of CREB protein 2 |
| Calculated Molecular Weight | 693 aa, 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC053562 |
| Gene Symbol | CRTC2 |
| Gene ID (NCBI) | 200186 |
| RRID | AB_2260910 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53ET0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRTC2, also named as TORC2, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. It acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. CRTC2 enhances the interaction of CREB1 with TAF4. Ir regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. CRTC2 regulates the expression of specific genes such as the steroidogenic gene, StAR. TORC2 was recently shown to be an important regulator of gluconeogenesis in the livers of mammals. It is one of the other key regulators of CRE-dependent MIE gene expression in NT2 cells. This regulation is linked to VIP-induced TORC2 dephosphorylation and translocation to the nucleus. (PMID: 19369332, 20504934 ) .
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRTC2,TORC2 antibody 12497-1-AP | Download protocol |
| IHC protocol for CRTC2,TORC2 antibody 12497-1-AP | Download protocol |
| IF protocol for CRTC2,TORC2 antibody 12497-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) cAMP-Induced Nuclear Condensation of CRTC2 Promotes Transcription Elongation and Cystogenesis in Autosomal Dominant Polycystic Kidney Disease.
| ||
Mol Cell LKB1 controls inflammatory potential through CRTC2-dependent histone acetylation | ||
Am J Transplant The tacrolimus-induced glucose homeostasis imbalance in terms of the liver: From bench to bedside. | ||
J Neurosci Clock and light regulation of the CREB coactivator CRTC1 in the suprachiasmatic circadian clock. | ||
J Virol Breaking human cytomegalovirus major immediate-early gene silence by vasoactive intestinal peptide stimulation of the protein kinase A-CREB-TORC2 signaling cascade in human pluripotent embryonal NTera2 cells. |
