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CRTAP Polyclonal antibody

CRTAP Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15724-1-AP

Print datasheet

Synonyms

cartilage associated protein, CASP, CRTAP, LEPREL3

验证数据展示

  • WB analysis using 15724-1-AP

    WB analysis using 15724-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 15724-1-AP

    IP experiment of HEK-293 using 15724-1-AP

    IP result of anti-CRTAP (IP:15724-1-AP, 4ug; Detection:15724-1-AP 1:500) with HEK-293 cells lysate 3000 ug.

  • IHC staining of human kidney using 15724-1-AP

    IHC staining of human kidney using 15724-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 15724-1-AP

    IHC staining of human kidney using 15724-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15724-1-AP

    IF Staining of HeLa using 15724-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CRTAP antibody (15724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue).

经过测试的应用

Positive WB detected inHT-1080 cells, human stomach tissue, NIH/3T3 cells, ROS1728 cells, HEK-293 cells, HeLa cells, human colon tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

15724-1-AP targets CRTAP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CRTAP fusion protein Ag8459 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cartilage associated protein
Synonyms cartilage associated protein, CASP, CRTAP, LEPREL3
Calculated Molecular Weight 46.5 kDa
Observed Molecular Weight 43-47 kDa
GenBank Accession NumberBC008745
Gene Symbol CRTAP
Gene ID (NCBI) 10491
RRIDAB_2261089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75718
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CRTAP antibody 15724-1-APDownload protocol
IHC protocol for CRTAP antibody 15724-1-APDownload protocol
IF protocol for CRTAP antibody 15724-1-APDownload protocol
IP protocol for CRTAP antibody 15724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Med Oncol

Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer

Authors - Liangshun Zhang
View Article