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验证数据展示

  • WB analysis using 15724-1-AP

    WB analysis using 15724-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 15724-1-AP

    IP experiment of HEK-293 using 15724-1-AP

    IP result of anti-CRTAP (IP:15724-1-AP, 4ug; Detection:15724-1-AP 1:500) with HEK-293 cells lysate 3000 ug.

  • IHC staining of human kidney using 15724-1-AP

    IHC staining of human kidney using 15724-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 15724-1-AP

    IHC staining of human kidney using 15724-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15724-1-AP

    IF Staining of HeLa using 15724-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CRTAP antibody (15724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue).

  • WB analysis of HeLa using 15724-1-AP

    WB analysis of HeLa using 15724-1-AP

    WB result of CRTAP antibody (15724-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRTAP transfected HeLa cells.

  • Featured Product
  • KD/KO验证

CRTAP Polyclonal antibody

CRTAP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15724-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Cartilage-associated protein, CASP, LEPREL3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-1080 cells, HEK-293 cells, HeLa cells, human colon tissue, human stomach tissue, NIH/3T3 cells, ROS1728 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15724-1-AP targets CRTAP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag8459

Product name: Recombinant human CRTAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-401 aa of BC008745

Sequence: HALDKYSGEHWAESVGYLEISLRLHRLLRDSEAFCHRNCSAAPQPEPAAGLASYPELRLFGGLLRRAHCLKRCKQGLPAFRQSQPSRDVLADFQRREPYKFLQFAYFKANNLPKAIAAAHTFLLKHPDDEMMKRNMAYYKSLPGAEDYIKDLETKSYESLFIRAVRAYNGENWRTSITDMELALPDFFKAFYECLAACEGSREIKDFKDFYLSIADHYVEVLECKIQCEENLTPVIGGYPVEKFVATMYHYLQFAYYKLNDLKNAAPCAVSYLLFDQNDKVMQQNLVYYQYHRDTWGLSDEHFQPRPEAVQFFNVTTLQKELYDFAKENIMDDDEGEVVEYVDDLLELEETS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cartilage associated protein
别名 Cartilage-associated protein, CASP, LEPREL3
计算分子量 46.5 kDa
观测分子量 43-47 kDa
GenBank蛋白编号BC008745
基因名称 CRTAP
Gene ID (NCBI) 10491
RRIDAB_2261089
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75718
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CRTAP antibody 15724-1-APDownload protocol
IHC protocol for CRTAP antibody 15724-1-APDownload protocol
IF protocol for CRTAP antibody 15724-1-APDownload protocol
IP protocol for CRTAP antibody 15724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Med Oncol

Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer

Authors - Liangshun Zhang
View Article
humanWB

Nat Commun

The structural basis for the collagen processing by human P3H1/CRTAP/PPIB ternary complex

Authors - Wenguo Li
View Article