验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
| Positive IHC detected in | mouse lung tissue, mouse brain tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13001-1-AP targets CRTAC1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3673 Product name: Recombinant human CRTAC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 99-451 aa of BC034245 Sequence: VYGNWNGPHRLYLQMSTHGKVRFRDIASPKFSMPSPVRTVITADFDNDQELEIFFNNIAYRSSSANRLFRVIRREHGDPLIEELNPGDALEPEGRGTGGVVTDFDGDGMLDLILSHGESMAQPLSVFRGNQGFNNNWLRVVPRTRFGAFARGAKVVLYTKKSGAHLRIIDGGSGYLCEMEPVAHFGLGKDEASSVEVTWPDGKMVSRNVASGEMNSVLEILYPRDEDTLQDPAPLECGQGFSQQENGHCMDTNECIQFPFVCPRDKPVCVNTYGSYRCRTNKKCSRGYEPNEDGTACVGTLGQSPGPRPTTPTAAAATAAAAAAAGAATAAPVLVDGDLNLGSVVKESCEPSC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cartilage acidic protein 1 |
| 别名 | ASPIC, ASPIC1, cartilage acidic protein 1, CEP 68, CEP68, CRTAC1 |
| 计算分子量 | 661 aa, 71 kDa |
| 观测分子量 | 68-72 kDa |
| GenBank蛋白编号 | BC034245 |
| 基因名称 | CRTAC1 |
| Gene ID (NCBI) | 55118 |
| RRID | AB_10638787 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NQ79 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CRTAC1 antibody 13001-1-AP | Download protocol |
| WB protocol for CRTAC1 antibody 13001-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Integrative single-cell meta-analysis reveals disease-relevant vascular cell states and markers in human atherosclerosis | ||
Sci Rep CRTAC1 identified as a promising diagnosis and prognostic biomarker in lung adenocarcinoma | ||
Mol Oncol A DIA-MS-based proteomics approach to find potential serum prognostic biomarkers in glioblastoma patients | ||
Am J Rhinol Allergy Analysis of CRSsNP Proteome Using a Highly Multiplexed Approach in Nasal Mucus |
