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验证数据展示

  • FC experiment of HepG2 using 84302-1-RR

    FC experiment of HepG2 using 84302-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CRLS1-Specific Recombinant antibody (84302-1-RR, Clone:241192G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 84302-1-RR

    WB analysis using 84302-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84302-1-RR (CRLS1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CRLS1-Specific Recombinant antibody

CRLS1-Specific Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84302-1-RR

产品说明书

CloneNo. 241192G7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

CRLS1, CLS, cardiolipin synthase 1, Cardiolipin synthase (CMP-forming), Cardiolipin synthase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, Jurkat cells, HepG2 cells, HL-60 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CRLS1 Ready-To-Use IHC Kit. CRLS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84302-1-RR targets CRLS1-Specific in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cardiolipin synthase 1
别名 CRLS1, CLS, cardiolipin synthase 1, Cardiolipin synthase (CMP-forming), Cardiolipin synthase
计算分子量 33 kDa
观测分子量32-35 kDa
GenBank蛋白编号NM_019095
基因名称 CRLS1
Gene ID (NCBI) 54675
RRIDAB_3671841
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UJA2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CRLS1-Specific antibody 84302-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols