• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86296-3-RR (same clone as 86296-3-PBS)

    WB analysis using 86296-3-RR (same clone as 86296-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86296-3-RR (CDK12/CRKRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 86296-3-RR (same clone as 86296-3-PBS)

    WB analysis of NIH/3T3 using 86296-3-RR (same clone as 86296-3-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86296-3-RR (CDK12/CRKRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 86296-3-RR (same clone as 86296-3-PBS)

    IF Staining of NIH/3T3 using 86296-3-RR (same clone as 86296-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CDK12/CRKRS antibody (86296-3-RR, Clone: 250880E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86296-3-RR (same clone as 86296-3-PBS)

    IF Staining of HeLa using 86296-3-RR (same clone as 86296-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK12/CRKRS antibody (86296-3-RR, Clone: 250880E5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86296-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86296-3-RR

    Affinity and Kinetic Characterization of 86296-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86296-3-RR against Human CDK12/CRKRS were performed. The affinity constant is 0.106 nM.

CDK12/CRKRS Recombinant monoclonal antibody, PBS Only

CDK12/CRKRS Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86296-3-PBS

产品说明书

CloneNo. 250880E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

CrkRS, CDC2 related protein kinase 7, Cdc2-related kinase, arginine/serine-rich, CDC2-related protein kinase 7, CDK12

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

产品信息

86296-3-PBS targets CDK12/CRKRS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag25312

Product name: Recombinant human CRKRS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1300-1481 aa of BC150265

Sequence: EAEPPGHLPHEHQALRPMEYSTRPRPNRTYGNTDGPETGFSAIDTDERNSGPALTESLVQTLVKNRTFSGSLSHLGESSSYQGTGSVQFPGDQDLRFARVPLALHPVVGQPFLKAEGSSNSVVHAETKLQNYGELGPGTTGASSSGAGLHWGGPTQSSAYGKLYRGPTRVPPRGGRGRGVPY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Cdc2-related kinase, arginine/serine-rich
别名 CrkRS, CDC2 related protein kinase 7, Cdc2-related kinase, arginine/serine-rich, CDC2-related protein kinase 7, CDK12
计算分子量 1490 aa, 164 kDa
观测分子量 180-200 kDa
GenBank蛋白编号BC150265
基因名称 CDK12
Gene ID (NCBI) 51755
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NYV4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CRKRS (Cdc2-related kinase, Arg/Ser), also named as cyclin-dependent kinase 12 (CKD12), belongs to CDC2/CDKX subfamily. CDK12 can form a heterodimeric complex with its activating partner, cyclin K, to regulate important cellular physiological processes that include transcriptional regulation, regulation of DNA damage repair genes such as BRCA1 and ATM, suppression of intronic polyadenylation, and susceptibility to PARP inhibitors (PMID: 30487607, 24240700, 31694772). CDK12 has 3 isoforms with the calculated molecular mass of 164/163/141 kDa, and the apparent molecular mass of 180-200 kDa due to some modifications (PMID 11683387, 22988298).

||
New chat Able
正在加载，请稍候...