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CRIPT Polyclonal antibody
CRIPT Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells |
| Positive IP detected in | L02 cells |
| Positive IF detected in | L02 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11211-1-AP targets CRIPT in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IP, WB, ELISA |
| Cited Applications | WB, IP, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Immunogen | CRIPT fusion protein Ag1708 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | cysteine-rich PDZ-binding protein |
| Synonyms | CRIPT, HSPC139 |
| Calculated Molecular Weight | 11 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC018653 |
| Gene Symbol | CRIPT |
| Gene ID (NCBI) | 9419 |
| RRID | AB_2084731 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P021 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The clustering and immobilization of receptors and ion channels located at specific subcellular sites are believed to be mediated by intracellular proteins that attach these membrane proteins to the cytoskeleton. Receptors and ion channels interact with cytoplasmic proteins, which involved in the modulation or downstream signaling mechanisms of the receptor/ion channel. CRIPT is the protein that binds to the third PDZ (PDZ3) domain of PSD95, which binds to and clusters a variety of membrane proteins, including Shaker-type potassium channel subunits and NMDA receptor NR2 subunits, via its first 2 N-terminal PDZ domains
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRIPT antibody 11211-1-AP | Download protocol |
| IF protocol for CRIPT antibody 11211-1-AP | Download protocol |
| IP protocol for CRIPT antibody 11211-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Neurobiol Interaction Between CRIPT and PSD-95 Is Required for Proper Dendritic Arborization in Hippocampal Neurons.
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