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验证数据展示

  • WB analysis using 85785-1-RR

    WB analysis using 85785-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85785-1-RR (CRHR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85785-1-RR

    WB analysis using 85785-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85785-1-RR (CRHR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CRHR2 Recombinant antibody

CRHR2 Recombinant Antibody for WB, ELISA
Cat No. 85785-1-RR

产品说明书

CloneNo. 250043F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Corticotropin-releasing factor receptor 2, Corticotropin-releasing hormone receptor 2, CRF R 2, CRF R2, CRF2R

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HL-60 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, HeLa cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85785-1-RR targets CRHR2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 corticotropin releasing hormone receptor 2
别名 Corticotropin-releasing factor receptor 2, Corticotropin-releasing hormone receptor 2, CRF R 2, CRF R2, CRF2R
计算分子量 48 kDa
观测分子量 48 kDa
GenBank蛋白编号NM_001883
基因名称 CRHR2
Gene ID (NCBI) 1395
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13324
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRHR2 is a receptor for corticotropin-releasing factor (CRF) and belongs to the G-protein coupled receptor family. It shows high affinity for CRF and also binds to related peptides such as urocortin I, II, and III. This receptor is involved in mediating the endocrine, autonomic, and behavioral responses to stress and immune challenges.

实验方案

Product Specific Protocols
WB protocol for CRHR2 antibody 85785-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols