• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CRHR2 Polyclonal antibody

CRHR2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 25267-1-AP

Print datasheet

Synonyms

CRF R 2, CRF R2, CRF2R, CRFR 2, CRFR2, CRH R 2, CRH R2, CRH2R, CRHR2

验证数据展示

  • WB analysis of rat skeletal muscle using 25267-1-AP

    WB analysis of rat skeletal muscle using 25267-1-AP

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25267-1-AP (CRHR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 25267-1-AP

    IHC staining of mouse brain using 25267-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25267-1-AP (CRHR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25267-1-AP

    IHC staining of mouse brain using 25267-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25267-1-AP (CRHR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat skeletal muscle tissue, mouse heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25267-1-AP targets CRHR2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name corticotropin releasing hormone receptor 2
Synonyms CRF R 2, CRF R2, CRF2R, CRFR 2, CRFR2, CRH R 2, CRH R2, CRH2R, CRHR2
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberNM_001883
Gene Symbol CRHR2
Gene ID (NCBI) 1395
RRIDAB_2879996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13324
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CRHR2 antibody 25267-1-APDownload protocol
IHC protocol for CRHR2 antibody 25267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Pharmaceuticals (Basel)

A Novel Mast Cell Stabilizer JM25-1 Rehabilitates Impaired Gut Barrier by Targeting the Corticotropin-Releasing Hormone Receptors

Authors - Yueshan Sun
View Article
mouseWB

Cell Signal

Activation of CRHR1 contributes to cerebral endothelial barrier impairment via cPLA2 phosphorylation in experimental ischemic stroke.

Authors - Changchun Cao
View Article
human,ratIHC,WB

Neurogastroenterol Motil

Lidocaine ameliorates intestinal barrier dysfunction in irritable bowel syndrome by modulating corticotropin-releasing hormone receptor 2

Authors - Yanrong Wang
View Article