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CoraLite® Plus 488-conjugated CRH/CRF Polyclonal antibody

CRH/CRF Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-10944

Print datasheet

Synonyms

Corticoliberin, Corticotropin releasing factor, CRF, CRH

验证数据展示

  • IF Staining of mouse brain using CL488-10944

    IF Staining of mouse brain using CL488-10944

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 CRH/CRF antibody (CL488-10944) at dilution of 1:200.

  • IF Staining of mouse brain using CL488-10944

    IF Staining of mouse brain using CL488-10944

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 CRH/CRF antibody (CL488-10944) at dilution of 1:200.

经过测试的应用

Positive IF detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 10944-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10944 targets CRH/CRF in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P
Tested Reactivity human, mouse, rat
Immunogen CRH/CRF fusion protein Ag1371 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CRH/CRF
Synonyms Corticoliberin, Corticotropin releasing factor, CRF, CRH
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC011031
Gene Symbol CRH
Gene ID (NCBI) 1392
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06850
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CRH/CRF antibody CL488-10944Download protocol
Standard Protocols
Click here to view our Standard Protocols