验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, rat brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10944-1-AP targets CRH/CRF in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, zebrafish, brandt’s voles, microtus ochrogaster |
| 免疫原 |
CatNo: Ag1371 Product name: Recombinant human CRH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-196 aa of BC011031 Sequence: MRLPLLVSAGVLLVALLPCPPCRALLSRGPVPGARQAPQHPQPLDFFQPPPQSEQPQQPQARPVLLRMGEEYFLRLGNLNKSPAAPLSPASSLLAGGSGSRPSPEQATANFFRVLLQQLLLPRRSLDSPAALAERGARNALGGHQEAPERERRSEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEIIGK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CRH/CRF |
| 别名 | Corticoliberin, CRF, CRH |
| 计算分子量 | 21 kDa |
| 观测分子量 | 35-38 kDa |
| GenBank蛋白编号 | BC011031 |
| 基因名称 | CRH |
| Gene ID (NCBI) | 1392 |
| RRID | AB_2084279 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P06850 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRH, also called CRF or corticoliberin, is a peptide hormone and neurotransmitter involved in the stress response. Marked reduction in this protein has been observed in association with Alzheimer disease. In addition to production in the hypothalamus, this protein is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta it is a marker that determines the length of gestation and the timing of parturition and delivery.
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res 40 Hz light flickering alleviates chronic pain via adenosine signaling in the retina-amygdala pathway. | ||
Cell Stem Cell Generation of human appetite-regulating neurons and tanycytes from pluripotent stem cells. | ||
Nat Commun Paraventricular hypothalamic RUVBL2 neurons suppress appetite by enhancing excitatory synaptic transmission in distinct neurocircuits | ||
J Adv Res Vitamin D/VDR regulates peripheral energy homeostasis via central renin-angiotensin system. | ||
Neuron Corticotropin-releasing hormone signaling from piriform cortex to amygdala mediates social homophily in opioid addiction | ||
J Exp Med Central neurons encode interleukin-1β signals and mediate stress-induced inflammation. |
