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验证数据展示

  • WB analysis of HepG2 using 29903-1-AP (same clone as 29903-1-PBS)

    WB analysis of HepG2 using 29903-1-AP (same clone as 29903-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

  • WB analysis of rat lung using 29903-1-AP (same clone as 29903-1-PBS)

    WB analysis of rat lung using 29903-1-AP (same clone as 29903-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 29903-1-AP (same clone as 29903-1-PBS)

    IF Staining of U2OS using 29903-1-AP (same clone as 29903-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CRELD1 antibody (29903-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse lung using 29903-1-AP (same clone as 29903-1-PBS)

    WB analysis of mouse lung using 29903-1-AP (same clone as 29903-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 29903-1-AP (CRELD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 29903-1-AP (same clone as 29903-1-PBS)

    IHC staining of mouse heart using 29903-1-AP (same clone as 29903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29903-1-AP (CRELD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29903-1-PBS in a different storage buffer formulation.

CRELD1 Polyclonal antibody, PBS Only

CRELD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29903-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

29903-1-PBS targets CRELD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag31593

Product name: Recombinant human CRELD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 218-362 aa of NM_001031717

Sequence: CARCSGPEESNCLQCKKGWALHHLKCVDIDECGTEGANCGADQFCVNTEGSYECRDCAKACLGCMGAGPGRCKKCSPGYQQVGSKCLDVDECETEVCPGENKQCENTEGGYRCICAEGYKQMEGICVKEQIPESAGFFSEMTEDE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cysteine-rich with EGF-like domains 1
别名
计算分子量45KD
观测分子量45 kDa, 70-75 kDa
GenBank蛋白编号NM_001031717
基因名称 CRELD1
Gene ID (NCBI) 78987
RRIDAB_2923619
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96HD1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CRELD1 (Cysteine-rich with EGF-like domain protein 1) belongs to matricellular proteins family. The encoded protein has a cysteine-rich with epidermal growth factor-like domain (PubMed: 12137942). Mutations in CRELD1 are the cause of atrioventricular septal defect (PubMed: 24697899). Alternate splicing results in multiple transcript variants. CRELD1 can be detected as 70-75 kDa in mouse lung (PMID: 24697899).

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