验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, mouse liver tissue, mouse ovary tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
19070-1-AP targets CREBZF in WB, IF, IHC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IF, IHC |
| 经测试反应性 | mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag13625 Product name: Recombinant human CREBZF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 185-301 aa of BC093796 Sequence: GLAAENQELRAENRELGKRVQALQEESRYLRAVLANETGLARLLSRLSGVGLRLTTSLFRDSPAGDHDYALPVGKQKQDLLEEDDSAGGVCLHVDKDKVSVEFCSACARKASSSLKM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CREB/ATF bZIP transcription factor |
| 别名 | CREBZF, SMILE, ZF, Zhangfei |
| 计算分子量 | 272 aa, 30 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC093796 |
| 基因名称 | CREBZF |
| Gene ID (NCBI) | 58487 |
| RRID | AB_10641036 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NS37 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CREBZF is a basic region-leucine zipper (bZIP) transcription factor that belongs to the cAMP-response-element-binding protein (CREB)/activating transcription factor (ATF) family. CREBZF gene expression produces two isoforms, CREBZF-L (long isoform of CREBZF, also known as SMILE) and CREBZF-S (short isoform of CREBZF, also known as Zhangfei), resulting from alternative initiation codons. CREBZF fulfills its role in transcriptional regulation by heterodimerizing with other transcription factors as a coregulator and then binding to target promoters and regulating downstream gene expression. CREBZF has been identified as a novel transcriptional coregulator of a variety of nuclear receptors (PMID: 31124138).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CREBZF antibody 19070-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |