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验证数据展示

  • WB analysis using 67617-1-Ig

    WB analysis using 67617-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of HepG2 using 67617-1-Ig

    FC experiment of HepG2 using 67617-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CREB3L1,OASIS (67617-1-Ig, Clone:2B5B4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • WB analysis using 67617-1-Ig

    WB analysis using 67617-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67617-1-Ig (CREB3L1,OASIS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CREB3L1,OASIS Monoclonal antibody

CREB3L1,OASIS Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67617-1-Ig

产品说明书

CloneNo. 2B5B4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

CREB3L1, OASIS, 2B5B4, cAMP-responsive element-binding protein 3-like protein 1, Cyclic AMP-responsive element-binding protein 3-like protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, A375 cells, A549 cells, 4T1 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CREB3L1 Ready-To-Use IHC Kit. CREB3L1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67617-1-Ig targets CREB3L1,OASIS in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28042

Product name: Recombinant human CREB3L1,OASIS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 170-519 aa of BC015781

Sequence: PHQAPGEMTQLPVIKAEPLEVNQFLKVTPEDLVQMPPTPPSSHGSDSDGSQSPRSLPPSSPVRPMARSSTAISTSPLLTAPHKLQGTSGPLLLTEEEKRTLIAEGYPIPTKLPLTKAEEKALKRVRRKIKNKISAQESRRKKKEYVECLEKKVETFTSENNELWKKVETLENANRTLLQQLQKLQTLVTNKISRPYKMAATQTGTCLMVAALCFVLVLGSLVPCLPEFSSGSQTVKEDPLAADGVYTASQMPSRSLLFYDDGAGLWEDGRSTLLPMEPPDGWEINPGGPAEQRPRDHLQHDHLDSTHETTKYLSEAWPKDGGNGTSPDFSHSKEWFHDRDLGPNTTIKLS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cAMP responsive element binding protein 3-like 1
别名 CREB3L1, OASIS, 2B5B4, cAMP-responsive element-binding protein 3-like protein 1, Cyclic AMP-responsive element-binding protein 3-like protein 1
计算分子量 57 kDa
观测分子量 58 kDa
GenBank蛋白编号BC015781
基因名称 CREB3L1/OASIS
Gene ID (NCBI) 90993
RRIDAB_2882820
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ96BA8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)

实验方案

Product Specific Protocols
FC protocol for CREB3L1,OASIS antibody 67617-1-IgDownload protocol
WB protocol for CREB3L1,OASIS antibody 67617-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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