验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, A375 cells, A549 cells, 4T1 cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67617-1-Ig targets CREB3L1,OASIS in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag28042 Product name: Recombinant human CREB3L1,OASIS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 170-519 aa of BC015781 Sequence: PHQAPGEMTQLPVIKAEPLEVNQFLKVTPEDLVQMPPTPPSSHGSDSDGSQSPRSLPPSSPVRPMARSSTAISTSPLLTAPHKLQGTSGPLLLTEEEKRTLIAEGYPIPTKLPLTKAEEKALKRVRRKIKNKISAQESRRKKKEYVECLEKKVETFTSENNELWKKVETLENANRTLLQQLQKLQTLVTNKISRPYKMAATQTGTCLMVAALCFVLVLGSLVPCLPEFSSGSQTVKEDPLAADGVYTASQMPSRSLLFYDDGAGLWEDGRSTLLPMEPPDGWEINPGGPAEQRPRDHLQHDHLDSTHETTKYLSEAWPKDGGNGTSPDFSHSKEWFHDRDLGPNTTIKLS 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cAMP responsive element binding protein 3-like 1 |
| 别名 | CREB3L1, OASIS, 2B5B4, cAMP-responsive element-binding protein 3-like protein 1, Cyclic AMP-responsive element-binding protein 3-like protein 1 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC015781 |
| 基因名称 | CREB3L1/OASIS |
| Gene ID (NCBI) | 90993 |
| RRID | AB_2882820 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96BA8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CREB3L1,OASIS antibody 67617-1-Ig | Download protocol |
| WB protocol for CREB3L1,OASIS antibody 67617-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
