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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67927-1-Ig (same clone as 67927-1-PBS)

    WB analysis using 67927-1-Ig (same clone as 67927-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67927-1-Ig (CREB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67927-1-Ig (same clone as 67927-1-PBS)

    WB analysis of HEK-293 using 67927-1-Ig (same clone as 67927-1-PBS)

    WB result of CREB1 antibody (67927-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREB1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 67927-1-Ig (same clone as 67927-1-PBS)

    IHC staining of human prostate cancer using 67927-1-Ig (same clone as 67927-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67927-1-Ig (CREB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CREB1 antibody included
  • IF Staining of A431 using 67927-1-Ig (same clone as 67927-1-PBS)

    IF Staining of A431 using 67927-1-Ig (same clone as 67927-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CREB1 antibody (67927-1-Ig, Clone: 1E11C1 ) at dilution of 1:8000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 67927-1-Ig (same clone as 67927-1-PBS)

    FC experiment of Jurkat using 67927-1-Ig (same clone as 67927-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.2 μg CREB1 Monoclonal antibody (67927-1-Ig, Clone:1E11C1,red) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005),Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA. This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 67927-1-Ig (same clone as 67927-1-PBS)

    FC experiment of HEK-293 using 67927-1-Ig (same clone as 67927-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug CREB1 Monoclonal antibody (67927-1-Ig, Clone:1E11C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67927-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CREB1 Monoclonal antibody, PBS Only

CREB1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67927-1-PBS

产品说明书

CloneNo. 1E11C1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

1E11C1, cAMP-responsive element-binding protein 1, CREB, CREB 1, CREB-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CREB1 Ready-To-Use IHC Kit. CREB1 primary antibody included.

产品信息

67927-1-PBS targets CREB1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CREB1 fusion protein Ag2852 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cAMP responsive element binding protein 1
别名 1E11C1, cAMP-responsive element-binding protein 1, CREB, CREB 1, CREB-1
计算分子量 341 aa, 35 kDa
观测分子量 43-46 kDa
GenBank蛋白编号BC010636
基因名称 CREB1
Gene ID (NCBI) 1385
RRIDAB_2918679
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16220
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CREB1, also named as CREB, belongs to the bZIP family, containing one bZIP domain and one KID (kinase-inducible) domain. This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. This protein is stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. CREB1 is sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization. Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH), a distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type. CREB1 exists some isoforms and range of calculated molecular weight of isoforms are 35-37 kDa and 25 kDa, but the modified CREB1 protein is about 43 kDa (PMID: 25883219 ).