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验证数据展示

  • IHC staining of mouse brain using 28015-1-AP

    IHC staining of mouse brain using 28015-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28015-1-AP (CRB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 28015-1-AP

    IF Staining of mouse eye using 28015-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CRB2 antibody (28015-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CRB2 Polyclonal antibody

CRB2 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 28015-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

Crumbs homolog 2, crumbs homolog 2 (Drosophila), Crumbs like protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28015-1-AP targets CRB2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27826

Product name: Recombinant human CRB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 102-282 aa of NM_173689

Sequence: MGPRCELDIDECASRPCHHGATCRNLADRYECHCPLGYAGVTCEMEVDECASAPCLHGGSCLDGVGSFRCVCAPGYGGTRCQLDLDECQSQPCAHGGTCHDLVNGFRCDCAGTGYEGTHCEREVLECASAPCEHNASCLEGLGSFRCLCWPGYSGELCEVDEDECASSPCQHGGRCLQRSDP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 crumbs homolog 2 (Drosophila)
别名 Crumbs homolog 2, crumbs homolog 2 (Drosophila), Crumbs like protein 2
计算分子量 134 aa
GenBank蛋白编号NM_173689
基因名称 CRB2
Gene ID (NCBI) 286204
RRIDAB_3669636
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5IJ48
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRB2 (Crumbs homolog-2) has a large extracellular domain with epidermal growth-factor-like and laminin-A globular domains, a single transmembrane domain, and an intracellular C-terminal domain of 37 amino acids (PMID: 31438467). CRB2 is a candidate modifying gene of human CRB1-related retinal dystrophy (PMID: 24565864).

实验方案

Product Specific Protocols
IF protocol for CRB2 antibody 28015-1-APDownload protocol
IHC protocol for CRB2 antibody 28015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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