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验证数据展示

  • WB analysis using 82012-2-RR (same clone as 82012-2-PBS)

    WB analysis using 82012-2-RR (same clone as 82012-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82012-2-RR (CPT1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82012-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82012-2-RR (same clone as 82012-2-PBS)

    IF Staining of HeLa using 82012-2-RR (same clone as 82012-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CPT1A antibody (82012-2-RR, Clone: 251085G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82012-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82012-2-RR (same clone as 82012-2-PBS)

    FC experiment of HeLa using 82012-2-RR (same clone as 82012-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CPT1A Recombinant monoclonal antibody (82012-2-RR, Clone:251085G11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 82012-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82012-2-RR

    Affinity and Kinetic Characterization of 82012-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82012-2-RR against Human CPT1A were performed. The affinity constant is 0.124 nM.

CPT1A Recombinant monoclonal antibody, PBS Only

CPT1A Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82012-2-PBS

产品说明书

CloneNo. 251085G11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

Carnitine O-palmitoyltransferase 1, liver isoform, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPT1, CPT-1A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® CPT1A Ready-To-Use IHC Kit. CPT1A primary antibody included.

产品信息

82012-2-PBS targets CPT1A in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7202

Product name: Recombinant human CPT1A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 406-756 aa of BC000185

Sequence: QSLDAVEKAAFFVTLDETEEGYRSEDPDTSMDSYAKSLLHGRCYDRWFDKSFTFVVFKNGKMGLNAEHSWADAPIVAHLWEYVMSIDSLQLGYAEDGHCKGDINPNIPYPTRLQWDIPGECQEVIETSLNTANLLANDVDFHSFPFVAFGKGIIKKCRTSPDAFVQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTTESCDFVRAMVDPAQTVEQRLKLFKLASEKHQHMYRLAMTGSGIDRHLFCLYVVSKYLAVESPFLKEVLSEPWRLSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGYGVSYILVGENLINFHISSKFSCPETGIISQGPSSDT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 carnitine palmitoyltransferase 1A (liver)
别名 Carnitine O-palmitoyltransferase 1, liver isoform, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPT1, CPT-1A
计算分子量 88 kDa
观测分子量86 kDa
GenBank蛋白编号BC000185
基因名称 CPT1A
Gene ID (NCBI) 1374
RRIDAB_3743399
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP50416
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CPT1A, also named CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. CPT1A gene is localized Chromosome 11q13.1-2. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active form of related liver-type carnitine palmitoyltransferase I (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.

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