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验证数据展示

  • WB analysis using 85203-1-RR

    WB analysis using 85203-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85203-1-RR (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 85203-1-RR

    IF Staining of A431 using 85203-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (85203-1-RR, Clone: 242644E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CPSF7 Recombinant antibody

CPSF7 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85203-1-RR

产品说明书

CloneNo. 242644E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Pre-mRNA cleavage factor Im 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, mouse uterus tissue
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CPSF7 Ready-To-Use IHC Kit. CPSF7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85203-1-RR targets CPSF7 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pre-mRNA cleavage factor I, 59 kDa subunit
别名 Pre-mRNA cleavage factor Im 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59
计算分子量 56 kDa
观测分子量52 kDa
GenBank蛋白编号NM_001136040
基因名称 CPSF7
Gene ID (NCBI) 79869
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N684
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.

实验方案

Product Specific Protocols
WB protocol for CPSF7 antibody 85203-1-RRDownload protocol
IF protocol for CPSF7 antibody 85203-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols