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验证数据展示

  • WB analysis using 85203-1-RR (same clone as 85203-1-PBS)

    WB analysis using 85203-1-RR (same clone as 85203-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85203-1-RR (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85203-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01956-1

    Sandwich ELISA standard curve of MP01956-1

    Sandwich ELISA standard curve of MP01956-1, Human CPSF7 Recombinant Matched Antibody Pair - PBS only. 85203-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01255. 85203-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

  • Sample test of MP01956-1

    Sample test of MP01956-1

    The mean CPSF7 concentration was determined to be 4,199.1 pg/mL in HEK-293 cell extract based on a 2.7 mg/mL extract load and 1,800.3 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • IF Staining of A431 using 85203-1-RR (same clone as 85203-1-PBS)

    IF Staining of A431 using 85203-1-RR (same clone as 85203-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (85203-1-RR, Clone: 242644E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85203-1-PBS in a different storage buffer formulation.

CPSF7 Recombinant antibody, PBS Only (Capture)

CPSF7 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85203-1-PBS

产品说明书

CloneNo. 242644E1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

别名

Pre-mRNA cleavage factor Im 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CPSF7 Ready-To-Use IHC Kit. CPSF7 primary antibody included.

产品信息

85203-1-PBS targets CPSF7 as part of a matched antibody pair:

MP01956-1: 85203-1-PBS capture and 85203-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pre-mRNA cleavage factor I, 59 kDa subunit
别名 Pre-mRNA cleavage factor Im 59 kDa subunit, Cleavage factor Im complex 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage and polyadenylation specificity factor 59 kDa subunit, CFIm59
计算分子量 56 kDa
观测分子量52 kDa
GenBank蛋白编号NM_001136040
基因名称 CPSF7
Gene ID (NCBI) 79869
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N684
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.