验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, human skin tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy CPSF7 Ready-To-Use IHC Kit. CPSF7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55195-1-AP targets CPSF7 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pre-mRNA cleavage factor I, 59 kDa subunit |
| 别名 | CFIm59, Cleavage and polyadenylation specificity factor 59 kDa subunit, Cleavage and polyadenylation specificity factor subunit 7, Cleavage factor Im complex 59 kDa subunit, Pre-mRNA cleavage factor Im 59 kDa subunit |
| 计算分子量 | 56 kDa |
| 观测分子量 | 52 kDa, 56 kDa |
| GenBank蛋白编号 | NM_001136040 |
| 基因名称 | CPSF7 |
| Gene ID (NCBI) | 79869 |
| RRID | AB_10858797 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N684 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CPSF7 antibody 55195-1-AP | Download protocol |
| IHC protocol for CPSF7 antibody 55195-1-AP | Download protocol |
| WB protocol for CPSF7 antibody 55195-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation | ||
J Mol Cell Biol U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors | ||
PLoS Pathog Downregulation of CPSF6 Leads to Global mRNA 3' UTR shortening and enhanced antiviral immune responses | ||
Cell Rep E3 ligase SYVN1-mediated polyubiquitination of CPSF6 promotes alternative polyadenylation and antivirus effects of macrophages | ||
Mol Cell Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors |
