验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cells, HEK-293 cells, HepG2 cells, human brain tissue, HeLa cells, Jurkat cells, NIH/3T3 cells, RAW 264.7 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse kidney tissue, human placenta tissue, human thyroid cancer tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
产品信息
11609-1-AP targets CPSF3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, pig |
| 免疫原 | CPSF3 fusion protein Ag2173 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cleavage and polyadenylation specific factor 3, 73kDa |
| 别名 | mRNA 3'-end-processing endonuclease CPSF-73, EC:3.1.27.-, CPSF73, CPSF 73 kDa subunit, CPSF 73 |
| 计算分子量 | 684 aa, 77 kDa |
| 观测分子量 | 77 kDa |
| GenBank蛋白编号 | BC020211 |
| 基因名称 | CPSF3 |
| Gene ID (NCBI) | 51692 |
| RRID | AB_2292188 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UKF6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CPSF3 (cleavage and polyadenylation specificity factor 3) is involved in human disease and is the putative target of benzoxaboroles in trypanosomatid parasites and Cryptosporidium infection. CPSF3 has also been associated with cancer malignancy in that CPSF3 induces cell cycle arrest in hepatocellular carcinoma and promotes lung adenocarcinoma cell proliferation and metastasis (PMID: 38718887). CPSF3 has been implicated in various cancers, including lung cancer, colorectal, prostate, and triple-negative breast cancers (PMID: 37627085).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CPSF3 antibody 11609-1-AP | Download protocol |
| IHC protocol for CPSF3 antibody 11609-1-AP | Download protocol |
| IP protocol for CPSF3 antibody 11609-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.
| ||
Cancers (Basel) CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma | ||
Cell Rep Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome. | ||
Cell Mol Life Sci Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs | ||
Cell Chem Biol Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3 | ||
Cell Discov RBBP6 maintains glioblastoma stem cells through CPSF3-dependent alternative polyadenylation
|
