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验证数据展示

  • WB analysis using 86513-3-RR (same clone as 86513-3-PBS)

    WB analysis using 86513-3-RR (same clone as 86513-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86513-3-RR (CPSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86513-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 86513-3-RR (same clone as 86513-3-PBS)

    IF Staining of HeLa using 86513-3-RR (same clone as 86513-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CPSF2 antibody (86513-3-RR, Clone: 251387H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86513-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86513-3-RR

    Affinity and Kinetic Characterization of 86513-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86513-3-RR against Human CPSF2 were performed. The affinity constant is 0.487 nM.

CPSF2 Recombinant monoclonal antibody, PBS Only

CPSF2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86513-3-PBS

产品说明书

CloneNo. 251387H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

CPSF 100 kDa subunit, CPSF100, KIAA1367

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CPSF2 Ready-To-Use IHC Kit. CPSF2 primary antibody included.

产品信息

86513-3-PBS targets CPSF2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11950

Product name: Recombinant human CPSF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 433-782 aa of BC070095

Sequence: SAHKTKHDLMMKGEGSRKGSFFKQAKKSYPMFPAPEERIKWDEYGEIIKPEDFLVPELQATEEEKSKLESGLTNGDEPMDQDLSDVPTKCISTTESIEIKARVTYIDYEGRSDGDSIKKIINQMKPRQLIIVHGPPEASQDLAECCRAFGGKDIKVYMPKLHETVDATSETHIYQVRLKDSLVSSLQFCKAKDAELAWIDGVLDMRVSKVDTGVILEEGELRDDGEDSEMQVEAPSDSSVIAQQKAMKSLFGDDEKETGEESEIIPTLEPLPPHEVPGHQSVFMNEPRLSDFKQVLLREGIQAEFVGGVLVCNNQVAVRRTETGRIGLEGCLCQDFYRIRDLLYEQYAIV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cleavage and polyadenylation specific factor 2, 100kDa
别名 CPSF 100 kDa subunit, CPSF100, KIAA1367
计算分子量 782 aa, 88 kDa
观测分子量 110 kDa
GenBank蛋白编号BC070095
基因名称 CPSF2
Gene ID (NCBI) 53981
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9P2I0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cleavage and Polyadenylation Specificity Factor Subunit 2 (CPSF2) is a critical component of the mRNA 3′ end processing machinery, playing a pivotal role in the cleavage and polyadenylation of pre-mRNA. This process is essential for the stability, nuclear export, and translation efficiency of mRNA, making CPSF2 a key player in gene expression regulation. Recent studies have highlighted the significance of CPSF2 in various diseases, particularly cancer. It's reported that in papillary thyroid carcinoma (PTC), negative expression of CPSF2 has been associated with poorer clinical outcomes, including higher rates of cervical lymph node metastasis, distant metastasis, and reduced recurrence-free survival. (PMID:26148673)

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