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验证数据展示

  • WB analysis of Jurkat using 11031-1-AP

    WB analysis of Jurkat using 11031-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 11031-1-AP

    WB analysis using 11031-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 11031-1-AP

    IHC staining of mouse testis using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 11031-1-AP

    IHC staining of mouse testis using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11031-1-AP

    IHC staining of mouse brain using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11031-1-AP

    IHC staining of mouse brain using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11031-1-AP

    IHC staining of mouse brain using 11031-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CPSF1 Polyclonal antibody

CPSF1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11031-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CPSF 160 kDa subunit, CPSF1, CPSF160, HSU37012, P/cl.18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, human brain tissue, HeLa cells
Positive IHC detected inmouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11031-1-AP targets CPSF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag1489

Product name: Recombinant human CPSF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1039-1443 aa of BC017232

Sequence: STNTPCARIPRMTGEEKEFETIERDERYIHPQQEAFSIQLISPVSWEAIPNARIELQEWEHVTCMKTVSLRSEETVSGLKGYVAAGTCLMQGEEVTCRGRILIMDVIEVVPEPGQPLTKNKFKVLYEKEQKGPVTALCHCNGHLVSAIGQKIFLWSLRASELTGMAFIDTQLYIHQMISVKNFILAADVMKSISLLRYQEESKTLSLVSRDAKPLEVYSVDFMVDNAQLGFLVSDRDRNLMVYMYLPEAKESFGGMRLLRRADFHVGAHVNTFWRTPCRGATEGLSKKSVVWENKHITWFATLDGGIGLLLPMQEKTYRRLLMLQNALTTMLPHHAGLNPRAFRMLHVDRRTLQNAVRNVLDGELLNRYLYLSTMERSELAKKIGTTPDIILDDLLETDRVTAHF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cleavage and polyadenylation specific factor 1, 160kDa
别名 CPSF 160 kDa subunit, CPSF1, CPSF160, HSU37012, P/cl.18
计算分子量 160 kDa
观测分子量 160 kDa
GenBank蛋白编号BC017232
基因名称 CPSF1
Gene ID (NCBI) 29894
RRIDAB_2245276
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ10570
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis.

实验方案

Product Specific Protocols
WB protocol for CPSF1 antibody 11031-1-APDownload protocol
IHC protocol for CPSF1 antibody 11031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
View Article
humanWB

J Biosci

Targeting cleavage and polyadenylation specific factor 1 via shRNA inhibits cell proliferation in human ovarian cancer.

Authors - Beiguang Zhang
  • KD Validated
View Article