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验证数据展示

  • WB analysis using 16724-1-AP

    WB analysis using 16724-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16724-1-AP

    IHC staining of human liver using 16724-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16724-1-AP

    IHC staining of human liver using 16724-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of K-562 using 16724-1-AP

    WB analysis of K-562 using 16724-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 16724-1-AP

    IP experiment of HepG2 using 16724-1-AP

    IP result of anti-CPOX (IP:16724-1-AP, 4ug; Detection:16724-1-AP 1:1000) with HepG2 cells lysate 1800ug.

CPOX Polyclonal antibody

CPOX Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 16724-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

Coprogen oxidase, Coproporphyrinogen oxidase, Coproporphyrinogenase, COX, CPO, CPOX, CPX, HCP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16724-1-AP targets CPOX in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10164

Product name: Recombinant human CPOX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 95-454 aa of BC017210

Sequence: HVQRAEMLPKTSGTRATSLGRPEEEEDELAHRCSSFMAPPVTDLGELRRRPGDMKTKMELLILETQAQVCQALAQVDGGANFSVDRWERKEGGGGISCVLQDGCVFEKAGVSISVVHGNLSEEAAKQMRSRGKVLKTKDGKLPFCAMGVSSVIHPKNPHAPTIHFNYRYFEVEEADGHKQWWFGGGCDLTPTYLNQEDAVHFHRTLKEACDQHGPDLYPKFKKWCDDYFFIAHRGERRGIGGIFFDDLDSPSKEEVFRFVQSCARAVVPSYIPLVKKHCDDSFTPQEKLWQQLRRGRYVEFNLLYDRGTKFGLFTPGSRIESILMSLPLTARWEYMHSPSENSKEAEILEVLRHPRDWVR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coproporphyrinogen oxidase
别名 Coprogen oxidase, Coproporphyrinogen oxidase, Coproporphyrinogenase, COX, CPO, CPOX, CPX, HCP
计算分子量 454 aa, 50 kDa
观测分子量 36 kDa
GenBank蛋白编号BC017210
基因名称 CPOX
Gene ID (NCBI) 1371
RRIDAB_2084231
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36551
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.

实验方案

Product Specific Protocols
WB protocol for CPOX antibody 16724-1-APDownload protocol
IHC protocol for CPOX antibody 16724-1-APDownload protocol
IP protocol for CPOX antibody 16724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols