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验证数据展示

  • WB analysis using 16724-1-AP

    WB analysis using 16724-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16724-1-AP

    IHC staining of human liver using 16724-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16724-1-AP

    IHC staining of human liver using 16724-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16724-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis of K-562 using 16724-1-AP

    WB analysis of K-562 using 16724-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 16724-1-AP (CPOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 16724-1-AP

    IP experiment of HepG2 using 16724-1-AP

    IP result of anti-CPOX (IP:16724-1-AP, 4ug; Detection:16724-1-AP 1:1000) with HepG2 cells lysate 1800ug.

CPOX Polyclonal antibody

CPOX Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 16724-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

COX, CPO, CPX, EC:1.3.3.3, HEM6

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, human liver tissue, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16724-1-AP targets CPOX in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10164

Product name: Recombinant human CPOX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 95-454 aa of BC017210

Sequence: HVQRAEMLPKTSGTRATSLGRPEEEEDELAHRCSSFMAPPVTDLGELRRRPGDMKTKMELLILETQAQVCQALAQVDGGANFSVDRWERKEGGGGISCVLQDGCVFEKAGVSISVVHGNLSEEAAKQMRSRGKVLKTKDGKLPFCAMGVSSVIHPKNPHAPTIHFNYRYFEVEEADGHKQWWFGGGCDLTPTYLNQEDAVHFHRTLKEACDQHGPDLYPKFKKWCDDYFFIAHRGERRGIGGIFFDDLDSPSKEEVFRFVQSCARAVVPSYIPLVKKHCDDSFTPQEKLWQQLRRGRYVEFNLLYDRGTKFGLFTPGSRIESILMSLPLTARWEYMHSPSENSKEAEILEVLRHPRDWVR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coproporphyrinogen oxidase
别名 COX, CPO, CPX, EC:1.3.3.3, HEM6
计算分子量 454 aa, 50 kDa
观测分子量 36 kDa
GenBank蛋白编号BC017210
基因名称 CPOX
Gene ID (NCBI) 1371
RRIDAB_2084231
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36551
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.

实验方案

Product Specific Protocols
IHC protocol for CPOX antibody 16724-1-APDownload protocol
IP protocol for CPOX antibody 16724-1-APDownload protocol
WB protocol for CPOX antibody 16724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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