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验证数据展示

  • WB analysis using 16075-1-AP

    WB analysis using 16075-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16075-1-AP (CPNE4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 16075-1-AP

    IF Staining of A549 using 16075-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CPNE4 antibody (16075-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

CPNE4 Polyclonal antibody

CPNE4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16075-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Copine 4, Copine 8, copine IV, COPN4, CPN4, CPNE4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16075-1-AP targets CPNE4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9037

Product name: Recombinant human CPNE4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 217-557 aa of BC014396

Sequence: LCSGDPDRRLKCIVWDWDSNGKHDFIGEFTSTFKEMRGAMEGKQVQWECINPKYKAKKKNYKNSGTVILNLCKIHKMHSFLDYIMGGCQIQFTVAIDFTASNGDPRNSCSLHYIHPYQPNEYLKALVAVGEICQDYDSDKMFPAFGFGARIPPEYTVSHDFAINFNEDNPECAGIQGVVEAYQSCLPKLQLYGPTNIAPIIQKVAKSASEETNTKEASQYFILLILTDGVITDMADTREAIVHASHLPMSVIIVGVGNADFSDMQMLDGDDGILRSPKGEPVLRDIVQFVPFRNFKHASPAALAKSVLAEVPNQVVDYYNGKGIKPKCSSEMYESSRTLAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 copine IV
别名 Copine 4, Copine 8, copine IV, COPN4, CPN4, CPNE4
计算分子量 557 aa, 62 kDa
观测分子量62 kDa
GenBank蛋白编号BC014396
基因名称 CPNE4
Gene ID (NCBI) 131034
RRIDAB_3085480
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96A23
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions. CPNE4 (Copine 4) belongs to the copine family. This antibody is specific to CPNE4.

实验方案

Product Specific Protocols
IF protocol for CPNE4 antibody 16075-1-APDownload protocol
WB protocol for CPNE4 antibody 16075-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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