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验证数据展示

  • IHC staining of mouse brain using 17664-1-AP

    IHC staining of mouse brain using 17664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17664-1-AP (CPNE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse brain using 17664-1-AP

    WB analysis of mouse brain using 17664-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17664-1-AP (CPNE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17664-1-AP

    IHC staining of mouse brain using 17664-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17664-1-AP (CPNE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CPNE2 Polyclonal antibody

CPNE2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17664-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CPN2, COPN2, Copine-2, Copine II, Copine 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17664-1-AP targets CPNE2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11946

Product name: Recombinant human CPNE2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 199-548 aa of BC053658

Sequence: IKYTLDPVWKPFTVPLVSLCDGDMEKPIQVMCYDYDNDGGHDFIGEFQTSVSQMCEARDSVPLEFECINPKKQRKKKNYKNSGIIILRSCKINRDYSFLDYILGGCQLMFTVGIDFTASNGNPLDPSSLHYINPMGTNEYLSAIWAVGQIIQDYDSDKMFPALGFGAQLPPDWKVSHEFAINFNPTNPFCSGVDGIAQAYSACLPHIRFYGPTNFSPIVNHVARFAAQATQQRTATQYFILLIITDGVISDMEETRHAVVQASKLPMSIIIVGVGNADFAAMEFLDGDSRMLRSHTGEEAARDIVQFVPFREFRNAAKETLAKAVLAELPQQVVQYFKHKNLPPTNSEPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 copine II
别名 CPN2, COPN2, Copine-2, Copine II, Copine 2
计算分子量 548 aa, 61 kDa
观测分子量61 kDa
GenBank蛋白编号BC053658
基因名称 CPNE2
Gene ID (NCBI) 221184
RRIDAB_3085531
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96FN4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.

实验方案

Product Specific Protocols
WB protocol for CPNE2 antibody 17664-1-APDownload protocol
IHC protocol for CPNE2 antibody 17664-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols