验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293T cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, mouse stomach tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
10126-2-AP targets CPNE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0139 Product name: Recombinant human CPNE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-289 aa of BC001142 Sequence: KSDPLCVLLQDVGGGSWAELGRTERVRNCSSPEFSKTLQLEYRFETVQKLRFGIYDIDNKTPELRDDDFLGGAECSLGQIVSSQVLTLPLMLKPGKPAGRGTITVSAQELKDNRVVTMEVEARNLDKKDFLGKSDPFLEFFRQGDGKWHLVYRSEVIKNNLNPTWKRFSVPVQHFCGGNPSTPIQVQCSDYDSDGSHDLIGTFHTSLAQLQAVPAEFECIHPEKQQKKKSYKNSGTIRVKICRVETEYSFLDYVMGGCQINFTVG 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | copine I |
| 别名 | Chromobindin 17, Copine 1, Copine I, Copine-1, copineI |
| 计算分子量 | 59 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC001142 |
| 基因名称 | CPNE1 |
| Gene ID (NCBI) | 8904 |
| RRID | AB_2084202 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99829 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CPNE1 antibody 10126-2-AP | Download protocol |
| WB protocol for CPNE1 antibody 10126-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Carcinogenesis Copine 1 predicts poor clinical outcomes by promoting M2 macrophage activation in ovarian cancer | ||
MedComm (2020) Hsa-miR-335 enhances cell migration and invasion in lung adenocarcinoma through targeting Copine-1. | ||
Front Biosci (Landmark Ed) Silencing of CPNE1-TRAF2 Axis Restrains the Development of Pancreatic Cancer
| ||
Cell Death Discov Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p |
