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验证数据展示

  • WB analysis of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    WB analysis of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66849-1-Ig (CPLX2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66849-1-Ig (CPLX2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    IHC staining of mouse brain using 66849-1-Ig (same clone as 66849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66849-1-Ig (CPLX2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP51525-1

    Cytometric bead array sample test of MP51525-1

    Cytometric bead array sample test of MP51525-1, CPLX2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66849-1-PBS. Detection antibody: 66849-2-PBS.

  • FC experiment of SH-SY5Y using 66849-1-Ig (same clone as 66849-1-PBS)

    FC experiment of SH-SY5Y using 66849-1-Ig (same clone as 66849-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug CPLX2 Monoclonal antibody (66849-1-Ig, Clone:1B2A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66849-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51525-1

    Cytometric bead array standard curve of MP51525-1

    Cytometric bead array standard curve of MP51525-1, CPLX2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66849-1-PBS. Detection antibody: 66849-2-PBS. Standard:Ag27949. Range: 3.125-400 ng/mL.

CPLX2 Monoclonal antibody, PBS Only (Capture)

CPLX2 Monoclonal Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 66849-1-PBS

产品说明书

CloneNo. 1B2A11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

1B2A11, 921 L, 921L, Complexin-2, CPLX 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66849-1-PBS targets CPLX2 as part of a matched antibody pair:

MP51525-1: 66849-1-PBS capture and 66849-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag27949

Product name: Recombinant human CPLX2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-134 aa of BC093706

Sequence: MDFVMKQALGGATKDMGKMLGGEEEKDPDAQKKEEERQEALRQQEEERKAKHARMEAEREKVRQQIRDKYGLKKKEEKEAEEKAALEQPCEGSLTRPKKAIPAGCGDEEEEEEESILDTVLKYLPGPLQDMFKK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 complexin 2
别名 1B2A11, 921 L, 921L, Complexin-2, CPLX 2
计算分子量 134 aa, 15 kDa
观测分子量 18-20 kDa
GenBank蛋白编号BC093706
基因名称 CPLX2
Gene ID (NCBI) 10814
RRIDAB_2882189
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ6PUV4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.

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