验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, PC-12 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50000-1:200000 |
| Immunofluorescence (IF)-P | IF-P : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
RMX00074 targets CPE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
FusionProtein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Multi-Recombinant |
| 产品类型 | Antibody |
| 全称 | carboxypeptidase E |
| 别名 | Carboxypeptidase E, Carboxypeptidase H, CPH, EC:3.4.17.10, Prohormone-processing carboxypeptidase |
| 计算分子量 | 53 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | NM_001873.4 |
| 基因名称 | Carboxypeptidase E |
| Gene ID (NCBI) | 1363 |
| RRID | AB_3750123 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | N/A |
| UNIPROT ID | P16870-1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CPE antibody RMX00074 | Download protocol |
| IHC protocol for CPE antibody RMX00074 | Download protocol |
| WB protocol for CPE antibody RMX00074 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
