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验证数据展示

  • WB analysis of HepG2 using 13604-1-AP

    WB analysis of HepG2 using 13604-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 13604-1-AP

    IHC staining of human gliomas using 13604-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 13604-1-AP

    IHC staining of human gliomas using 13604-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 13604-1-AP

    IF Staining of HepG2 using 13604-1-AP

    Immunofluorescent analysis of HepG2 cells using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Carboxypeptidase A6 Polyclonal antibody

Carboxypeptidase A6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13604-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:3.4.17.-, CPA6, Carboxypeptidase B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, mouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13604-1-AP targets Carboxypeptidase A6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag4466

Product name: Recombinant human CPA6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 130-437 aa of BC033684

Sequence: SLSGYNYEVYHSLEEIQNWMHHLNKTHSGLIHMFSIGRSYEGRCLFILKLGRRSRLKRAVWIDCGIHAREWIGPAFCQWFVKEALLTYKSDPAMRKMLNHLYFYIMPVFNVDGYHFSWTNDRFWRKTRSRNSRFRCRGVDANRNWKVKWCDEGASMHPCDDTYCGPFPESEPEVKAVANFLRKHRKHIRAYLSFHAYAQMLLYPYSYKYATIPNFRCVESAAYKAVNALQSVYGVRYRYGPASTTLYVSSGSSMDWAYKNGIPYAFAFELRDTGYFGFLLPEMLIKPTCTETMLAVKNITMHLLKKCP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carboxypeptidase A6
别名 EC:3.4.17.-, CPA6, Carboxypeptidase B
计算分子量 437 aa, 51 kDa
观测分子量 65 kDa, 51 kDa
GenBank蛋白编号BC033684
基因名称 CPA6
Gene ID (NCBI) 57094
RRIDAB_2261427
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N4T0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carboxypeptidase A6 (CPA6) is an extracellular matrixbound metallocarboxypeptidase (CP) that has been implicated in Duane syndrome, a neurodevelopmental disorder in which the lateral rectus extraocularmuscle is not properly innervated.Consistent with a role inDuane syndrome, CPA6 is expressed in a number of chondrocytic and nervous tissues during embryogenesis.It has three isoforms.The product recognizes the C-terminal of this protein.

实验方案

Product Specific Protocols
IF protocol for Carboxypeptidase A6 antibody 13604-1-APDownload protocol
IHC protocol for Carboxypeptidase A6 antibody 13604-1-APDownload protocol
WB protocol for Carboxypeptidase A6 antibody 13604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Arch Biochem Biophys

Carboxypeptidase A6 suppresses the proliferation and invasion of colorectal cancer cells and is negatively regulated by miR-96-3p

Authors - Xianren Wang
View Article
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