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验证数据展示

  • WB analysis using 80965-5-RR

    WB analysis using 80965-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80965-5-RR (CP110 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 80965-5-RR

    IHC staining of mouse testis using 80965-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80965-5-RR (CP110 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CP110 antibody included
  • IF Staining of HepG2 using 80965-5-RR

    IF Staining of HepG2 using 80965-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CP110 antibody (80965-5-RR, Clone: 242016E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80965-5-RR

    Affinity and Kinetic Characterization of 80965-5-RR

    Biolayer interferometry (BLl) kinetic assays of 80965-5-RR against Human CP110 were performed. The affinity constant is below 1 pM.

CP110 Recombinant monoclonal antibody

CP110 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 80965-5-RR

产品说明书

CloneNo. 242016E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CP110 protein, CP 110, Cep110, Centriolar coiled-coil protein of 110 kDa, CCP110

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, Jurkat cells, MOLT-4 cells, HCT 116 cells, HeLa cells, K-562 cells, U2OS cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CP110 Ready-To-Use IHC Kit. CP110 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80965-5-RR targets CP110 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3489

Product name: Recombinant human CP110 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-337 aa of BC036654

Sequence: MEEYEKFCEKSLARIQEASLSTESFLPAQSESISLIRFHGVAILSPLLNIEKRKEMQQEKQKALDVEARKQVNRKKALLTRVQEILDNVQVRKAPNASDFDQWEMETVYSNSEVRNLNVPATFPNSFPSHTEHSTAAKLDKIAGILPLDNEDQCKTDGIDLARDSEGFNSPKQCDSSNISHVENEAFPKTSSATPQETLISDGPFSVNEQQDLPLLAEVIPDPYVMSLQNLMKKSKEYIEREQSRRSLRGSMNRIVNESHLDKEHDAVEVADCVKEKGQLTGKHCVSVIPDKPSLNKSNVLLQGASTQASSMSMPVLASFSKVDIPIRTGHPTVLES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CP110 protein
别名 CP110 protein, CP 110, Cep110, Centriolar coiled-coil protein of 110 kDa, CCP110
计算分子量 991 aa, 111 kDa
观测分子量 113 kDa
GenBank蛋白编号BC036654
基因名称 CP110
Gene ID (NCBI) 9738
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43303
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.

实验方案

Product Specific Protocols
IF protocol for CP110 antibody 80965-5-RRDownload protocol
IHC protocol for CP110 antibody 80965-5-RRDownload protocol
WB protocol for CP110 antibody 80965-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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