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验证数据展示

  • WB analysis using 80965-5-RR (same clone as 80965-5-PBS)

    WB analysis using 80965-5-RR (same clone as 80965-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80965-5-RR (CP110 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80965-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01447-3

    Sandwich ELISA standard curve of MP01447-3

    Sandwich ELISA standard curve of MP01447-3, Human CP110 Recombinant Matched Antibody Pair - PBS only. 80965-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3489. 80965-5-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL

  • Sample test of MP01447-3

    Sample test of MP01447-3

    The mean CP110 concentration was determined to be 31.64 ng/mL in Jurkat cell extract based on a 4.2 mg/mL extract load and 9.42 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

  • IHC staining of mouse testis using 80965-5-RR (same clone as 80965-5-PBS)

    IHC staining of mouse testis using 80965-5-RR (same clone as 80965-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80965-5-RR (CP110 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80965-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CP110 antibody included
  • IF Staining of HepG2 using 80965-5-RR (same clone as 80965-5-PBS)

    IF Staining of HepG2 using 80965-5-RR (same clone as 80965-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CP110 antibody (80965-5-RR, Clone: 242016E10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80965-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80965-5-RR

    Affinity and Kinetic Characterization of 80965-5-RR

    Biolayer interferometry (BLl) kinetic assays of 80965-5-RR against Human CP110 were performed. The affinity constant is below 1 pM.

CP110 Recombinant monoclonal antibody, PBS Only (Detector)

CP110 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 80965-5-PBS

产品说明书

CloneNo. 242016E10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

别名

CP110 protein, CP 110, Cep110, Centriolar coiled-coil protein of 110 kDa, CCP110

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CP110 Ready-To-Use IHC Kit. CP110 primary antibody included.

产品信息

80965-5-PBS targets CP110 as part of a matched antibody pair:

MP01447-3: 80965-4-PBS capture and 80965-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

CatNo: Ag3489

Product name: Recombinant human CP110 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-337 aa of BC036654

Sequence: MEEYEKFCEKSLARIQEASLSTESFLPAQSESISLIRFHGVAILSPLLNIEKRKEMQQEKQKALDVEARKQVNRKKALLTRVQEILDNVQVRKAPNASDFDQWEMETVYSNSEVRNLNVPATFPNSFPSHTEHSTAAKLDKIAGILPLDNEDQCKTDGIDLARDSEGFNSPKQCDSSNISHVENEAFPKTSSATPQETLISDGPFSVNEQQDLPLLAEVIPDPYVMSLQNLMKKSKEYIEREQSRRSLRGSMNRIVNESHLDKEHDAVEVADCVKEKGQLTGKHCVSVIPDKPSLNKSNVLLQGASTQASSMSMPVLASFSKVDIPIRTGHPTVLES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CP110 protein
别名 CP110 protein, CP 110, Cep110, Centriolar coiled-coil protein of 110 kDa, CCP110
计算分子量 991 aa, 111 kDa
观测分子量 113 kDa
GenBank蛋白编号BC036654
基因名称 CP110
Gene ID (NCBI) 9738
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43303
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.

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