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验证数据展示

  • WB analysis of HepG2 using 82916-1-RR (same clone as 82916-1-PBS)

    WB analysis of HepG2 using 82916-1-RR (same clone as 82916-1-PBS)

    WB result of COXIV antibody (82916-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COXIV transfected HepG2 cells. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • WB analysis using 82916-1-RR (same clone as 82916-1-PBS)

    WB analysis using 82916-1-RR (same clone as 82916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 82916-1-RR (same clone as 82916-1-PBS)

    IHC staining of human stomach using 82916-1-RR (same clone as 82916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • WB analysis using 82916-1-RR (same clone as 82916-1-PBS)

    WB analysis using 82916-1-RR (same clone as 82916-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82916-1-RR (COXIV antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 82916-1-RR (same clone as 82916-1-PBS)

    IHC staining of human colon using 82916-1-RR (same clone as 82916-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82916-1-RR (COXIV antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82916-1-RR (same clone as 82916-1-PBS)

    IF Staining of HepG2 using 82916-1-RR (same clone as 82916-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COXIV antibody (82916-1-RR, Clone: 3K20 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82916-1-RR (same clone as 82916-1-PBS)

    FC experiment of HeLa using 82916-1-RR (same clone as 82916-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug COXIV Recombinant antibody (82916-1-RR, Clone:3K20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82916-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82916-1-RR

    Affinity and Kinetic Characterization of 82916-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82916-1-RR against Human COXIV were performed. The affinity constant is 9.41 nM.

  • Featured Product
  • KD/KO验证

COXIV Recombinant monoclonal antibody, PBS Only

COXIV Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82916-1-PBS

产品说明书

CloneNo. 3K20

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

COX4I1, 3K20, COX IV-1, COX4, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82916-1-PBS targets COXIV in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1640

Product name: Recombinant human COXIV protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-169 aa of BC021236

Sequence: MLATRVFSLVGKRAISTSVCVRAHESVVKSEDFSLPAYMDRRDHPLPEVAHVKHLSASQKALKEKEKASWSSLSMDEKVELYRIKFKESFAEMNRGSNEWKTVVGGAMFFIGFTALVIMWQKHYVYGPLPQSFDKEWVAKQTKRMLDMKVNPIQGLASKWDYEKNEWKK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome c oxidase subunit IV isoform 1
别名 COX4I1, 3K20, COX IV-1, COX4, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
计算分子量19.6 kDa
观测分子量17-18 kDa
GenBank蛋白编号BC021236
基因名称 COX IV
Gene ID (NCBI) 1327
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13073
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues.

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