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验证数据展示

  • WB analysis using 11437-1-AP

    WB analysis using 11437-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11437-1-AP (COX6B2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 11437-1-AP

    IHC staining of mouse testis using 11437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11437-1-AP (COX6B2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 11437-1-AP

    IHC staining of mouse testis using 11437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11437-1-AP (COX6B2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COX6B2 Polyclonal antibody

COX6B2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11437-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

COX VIb-2, Cytochrome c oxidase subunit 6B2, Cytochrome c oxidase subunit VIb, testis-specific isoform

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11437-1-AP targets COX6B2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原

CatNo: Ag1983

Product name: Recombinant human COX6B2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-88 aa of BC064548

Sequence: MLDVEAQEPPKGKWSTPPFDPRFPSQNQIRNCYQNFLDYHRCLKTRTRRGKSTQPCEYYFRVYHSLCPISWVESWNEQIKNGIFAGKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome c oxidase subunit VIb polypeptide 2 (testis)
别名 COX VIb-2, Cytochrome c oxidase subunit 6B2, Cytochrome c oxidase subunit VIb, testis-specific isoform
计算分子量 88 aa, 11 kDa
观测分子量 11 kDa
GenBank蛋白编号BC064548
基因名称 COX6B2
Gene ID (NCBI) 125965
RRIDAB_10859092
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6YFQ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX6B2(Cytochrome c oxidase subunit 6B2) encodes the testis-specific cytochrome C oxidase subunit VIb. It has been shown to be expressed in a subset of lung and breast tumors at a level >1% of the testicular level of expression(PMID: 24491090). It belongs to the cytochrome c oxidase subunit 6B family.

实验方案

Product Specific Protocols
WB protocol for COX6B2 antibody 11437-1-APDownload protocol
IHC protocol for COX6B2 antibody 11437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

PMID: 20870728

Authors - Oliva Claudia R CR
View Article
mouseWB

Cell Death Dis

TMEM232 is required for the formation of sperm flagellum and male fertility in mice

Authors - Xinying Cai
View Article