• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86783-3-RR

    WB analysis using 86783-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86783-3-RR (COX6B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 86783-3-RR

    IF Staining of HeLa using 86783-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX6B1 antibody (86783-3-RR, Clone: 251743G4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86783-3-RR

    Affinity and Kinetic Characterization of 86783-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86783-3-RR against Human COX6B1 were performed. The affinity constant is 1.18 nM.

COX6B1 Recombinant monoclonal antibody

COX6B1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86783-3-RR

产品说明书

CloneNo. 251743G4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

COX VIb 1, COX VIb-1, COX6B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, Caco-2 cells, HeLa cells, human heart tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy® COX6B1 Ready-To-Use IHC Kit. COX6B1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86783-3-RR targets COX6B1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1984

Product name: Recombinant human COX6B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-86 aa of BC001015

Sequence: MAEDMETKIKNYKTAPFDSRFPNQNQTRNCWQNYLDFHRCQKAMTAKGGDISVCEWYQRVYQSLCPTSWVTDWDEQRAEGTFPGKI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome c oxidase subunit Vib polypeptide 1 (ubiquitous)
别名 COX VIb 1, COX VIb-1, COX6B
计算分子量 80 aa, 10 kDa
观测分子量10-13 kDa
GenBank蛋白编号BC001015
基因名称 COX6B1
Gene ID (NCBI) 1340
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP14854
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX6B1 (Cytochrome c oxidase subunit 6B1) is also named as COX6B and belongs to the cytochrome c oxidase subunit 6B family. It connects the two COX monomers into the physiological dimeric form. Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D).

实验方案

Product Specific Protocols
IF protocol for COX6B1 antibody 86783-3-RRDownload protocol
WB protocol for COX6B1 antibody 86783-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat

Able™

正在加载，请稍候...