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验证数据展示

  • IF Staining of HepG2 using 18071-1-AP

    IF Staining of HepG2 using 18071-1-AP

    Immunofluorescent analysis of HepG2 cells, using COX18 antibody 18071-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis of mouse liver using 18071-1-AP

    WB analysis of mouse liver using 18071-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18071-1-AP (COX18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 18071-1-AP

    IHC staining of human ovary cancer using 18071-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18071-1-AP (COX18 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COX18 Polyclonal antibody

COX18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 18071-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

OXA1L2, Cytochrome c oxidase assembly protein COX18, mitochondrial, COX18Hs

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

18071-1-AP targets COX18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag12709

Product name: Recombinant human COX18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 187-333 aa of BC101684

Sequence: ALRNLSTGAAHSEGFSVQEQLATGGILWFPDLTAPDSTWILPISVGVINLLIVEICALQKIGMSRFQTYITYFVRAMSVLMIPIAATVPSSIVLYWLCSSFVGLSQNLLLRSPGFRQLCRIPSTKSDSETPYKDIFAAFNTKFISRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 COX18 cytochrome c oxidase assembly homolog (S. cerevisiae)
别名 OXA1L2, Cytochrome c oxidase assembly protein COX18, mitochondrial, COX18Hs
计算分子量 333 aa, 37 kDa
观测分子量 37-40 kDa, 28-30 kDa
GenBank蛋白编号BC101684
基因名称 COX18
Gene ID (NCBI) 285521
RRIDAB_10638443
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N8Q8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for COX18 antibody 18071-1-APDownload protocol
IHC protocol for COX18 antibody 18071-1-APDownload protocol
WB protocol for COX18 antibody 18071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Stem Cell

Selective translation of nuclear mitochondrial respiratory proteins reprograms succinate metabolism in AML development and chemoresistance

Authors - Guoqiang Han
View Article
humanWB

Biochim Biophys Acta

The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis.

Authors - Isotta Lorenzi
View Article
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