A431 cells were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COTL1 antibody (60237-1-Ig, Clone: 4A3D9 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

WB result of COTL1 antibody (60237-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COTL1 transfected HeLa cells. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.