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验证数据展示

  • WB analysis of Jurkat using 13802-1-AP

    WB analysis of Jurkat using 13802-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13802-1-AP (CORO2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 13802-1-AP

    IHC staining of human ovary cancer using 13802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13802-1-AP (CORO2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 13802-1-AP

    IHC staining of human ovary cancer using 13802-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 13802-1-AP (CORO2B antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CORO2B Polyclonal antibody

CORO2B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13802-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Protein FC96, KIAA0925, Coronin-like protein C, Coronin-2B, Coronin 2B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman ovary cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13802-1-AP targets CORO2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4793

Product name: Recombinant human CORO2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-331 aa of BC026335

Sequence: MSWRPQYRSSKFRNVYGKVANREHCFDGIPITKNVHDNHFCAVNTRFLAIVTESAGGGSFLVIPLEQTGRIEPNYPKVCGHQGNVLDIKWNPFIDNIIASCSEDTSVRIWEIPEGGLKRNMTEALLELHGHSRRVGLVEWHPTTNNILFSAGYDYKVLIWNLDVGEPVKMIDCHTDVILCMSFNTDGSLLTTTCKDKKLRVIEPRSGRVLQEANCKNHRVNRVVFLGNMKRLVTTGVSRWNTRQIALWDQEDLSMPLIEEEIDGLSGLLFPFYDADTHMLYLAGKGDGNIRYYEISTEKPYLSYLMEFRSPAPQKGLGVMPKHGLDVSACE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coronin, actin binding protein, 2B
别名 Protein FC96, KIAA0925, Coronin-like protein C, Coronin-2B, Coronin 2B
计算分子量 475 aa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC026335
基因名称 CORO2B
Gene ID (NCBI) 10391
RRIDAB_2087042
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQ03
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for CORO2B antibody 13802-1-APDownload protocol
WB protocol for CORO2B antibody 13802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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