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验证数据展示

  • WB analysis using 10119-1-AP (same clone as 10119-1-PBS)

    WB analysis using 10119-1-AP (same clone as 10119-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10119-1-AP (CORO2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10119-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 10119-1-AP (same clone as 10119-1-PBS)

    IHC staining of human skin cancer using 10119-1-AP (same clone as 10119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 10119-1-AP (CORO2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10119-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 10119-1-AP (same clone as 10119-1-PBS)

    WB analysis of Jurkat using 10119-1-AP (same clone as 10119-1-PBS)

    WB result of CORO2A antibody (10119-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CORO2A transfected Jurkat cells. This data was developed using the same antibody clone with 10119-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CORO2A Polyclonal antibody, PBS Only

CORO2A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10119-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10119-1-PBS targets CORO2A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0164

Product name: Recombinant human CORO2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 293-523 aa of BC000010

Sequence: GNIRYYEVSADKPHLSYLTEYRSYNPQKGIGVMPKRGLDVSSCEIFRFYKLITTKSLIEPISMIVPRRSESYQEDIYPPTAGAQPSLTAQEWLSGMNRDPILVSLRPGSELLRPHPLPAERPIFNSMAPASPRLLNQTEKLAAEDGWRSSSLLEEKMPRWAAEHRLEEKKTWLTNGFDVFECPPPKTENELLQMFYRQQEEIRRLRELLTQREVQAKQLELEIKNLRMGSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coronin, actin binding protein, 2A
别名
计算分子量 60 kDa
观测分子量 48 kDa
GenBank蛋白编号BC000010
基因名称 CORO2A
Gene ID (NCBI) 7464
RRIDAB_2877724
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92828
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CORO2A (Coronin 2A), also named as CLIPINB, is a member of the 'short' coronin subfamily containing a single WD40-repeat domain. It has been shown that the expression of CORO2A is up-regulated in human colon cancer. CORO2A can interact with MAPK14 and PRMT5. The calculated MW of CORO2A is 60 kDa, while 10119-1-AP antibody detects 48 kDa protein which is similar to the paper published. (PMID: 26373535)

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