验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy CORO1A Ready-To-Use IHC Kit. CORO1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84602-5-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-84602-5 targets CORO1A in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | CORO1A fusion protein Ag12139 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | coronin, actin binding protein, 1A |
| 别名 | 242027H5, CLABP, Clipin A, CLIPINA, Clipin-A |
| 计算分子量 | 461 aa, 51 kDa |
| 观测分子量 | 57 kDa |
| GenBank蛋白编号 | BC110374 |
| 基因名称 | CORO1A |
| Gene ID (NCBI) | 11151 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P31146 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CORO1A also known as TACO, belongs to the WD repeat coronin family. CORO1A is recruited to phagosomes and actively retained by surviving mycobacteria and is usually released before phagosome fusion with or maturation into lysosomes. CORO1A is expressed in the brain, thymus, spleen, bone marrow, and lymph nodes. Mutations in CORO1A cause Immunodeficiency 8 with lymphoproliferation (IMD8)(PMID: 10338208).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CORO1A antibody CL488-84602-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |