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验证数据展示

  • WB analysis of human heart using 67162-1-Ig

    WB analysis of human heart using 67162-1-Ig

    human heart tissue were subjected to SDS PAGE followed by western blot with 67162-1-Ig (COQ6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat heart using 67162-1-Ig

    IHC staining of rat heart using 67162-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67162-1-Ig (COQ6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat heart using 67162-1-Ig

    IHC staining of rat heart using 67162-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67162-1-Ig (COQ6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COQ6 Monoclonal antibody

COQ6 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67162-1-Ig

产品说明书

CloneNo. 2F5B1

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, ELISA

别名

CGI 10, COQ6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, mouse brain tissue, pig brain tissue, pig heart tissue, rat heart tissue
Positive IHC detected inrat heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67162-1-Ig targets COQ6 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 COQ6 fusion protein Ag28991 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 coenzyme Q6 homolog, monooxygenase (S. cerevisiae)
别名 CGI 10, COQ6
计算分子量 468 aa, 51 kDa
观测分子量 51 kDa
GenBank蛋白编号BC014181
基因名称 COQ6
Gene ID (NCBI) 51004
RRIDAB_2882458
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y2Z9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COQ6 antibody 67162-1-IgDownload protocol
IHC protocol for COQ6 antibody 67162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols