验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue, mouse liver tissue, rat liver tissue, mouse skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85890-1-RR targets COQ5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | COQ5 fusion protein Ag10203 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | coenzyme Q5 homolog, methyltransferase (S. cerevisiae) |
| 别名 | 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase, mitochondrial, EC:2.1.1.201, Ubiquinone biosynthesis methyltransferase COQ5 |
| 计算分子量 | 327 aa, 37 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC107874 |
| 基因名称 | COQ5 |
| Gene ID (NCBI) | 84274 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q5HYK3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COQ5 catalyzes the only C-methylation involved in the biosynthesis of coenzyme Q (Q or ubiquinone) in humans and yeast Saccharomyces cerevisiae. In humans, mutations in several COQ genes cause primary Q deficiency, and a decrease in Q biosynthesis is associated with mitochondrial, cardiovascular, kidney and neurodegenerative diseases (PMID: 25152161). Endogenous human COQ5 protein primarily existed as a mature form (m-COQ5, ~32 kDa) without the mitochondrial targeting sequence (MTS) in the mitochondria of human cells, in addition to the minor precursor form (p-COQ5, 37 kDa) of the full-length protein (PMID: 27155576).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COQ5 antibody 85890-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |