验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, mouse brain tissue, mouse liver tissue |
Positive IHC detected in | human heart tissue, human brain tissue, human kidney tissue, human liver tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 8 publications below |
IF | See 1 publications below |
产品信息
17453-1-AP targets COQ5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | COQ5 fusion protein Ag10203 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | coenzyme Q5 homolog, methyltransferase (S. cerevisiae) |
别名 | COQ5 |
计算分子量 | 327 aa, 37 kDa |
观测分子量 | 34.7 kDa |
GenBank蛋白编号 | BC107874 |
基因名称 | COQ5 |
Gene ID (NCBI) | 84274 |
RRID | AB_10547974 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q5HYK3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for COQ5 antibody 17453-1-AP | Download protocol |
IHC protocol for COQ5 antibody 17453-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
EMBO Mol Med β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice. | ||
Redox Biol The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency. | ||
J Am Soc Nephrol ADCK4 Deficiency Destabilizes the Coenzyme Q Complex, Which Is Rescued by 2,4-Dihydroxybenzoic Acid Treatment. | ||
EMBO Mol Med The clinical heterogeneity of coenzyme Q10 deficiency results from genotypic differences in the Coq9 gene. | ||
Sci Rep Reduction in the levels of CoQ biosynthetic proteins is related to an increase in lifespan without evidence of hepatic mitohormesis. |