验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29496-1-AP targets COQ10A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | COQ10A fusion protein Ag31534 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | coenzyme Q10 homolog A (S. cerevisiae) |
| 别名 | Coenzyme Q-binding protein COQ10 homolog A, mitochondrial |
| 计算分子量 | 28KD |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | NM_144576 |
| 基因名称 | COQ10A |
| Gene ID (NCBI) | 93058 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96MF6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COQ10A is a human gene that encodes a protein involved in the biosynthesis of coenzyme Q10 (CoQ10), also known as ubiquinone. CoQ10 is a lipid-soluble antioxidant and a critical component of the mitochondrial electron transport chain, where it plays a key role in cellular energy production (ATP synthesis). Mutations or deficiencies in COQ10A have been implicated in primary CoQ10 deficiency syndromes, which can manifest as mitochondrial disorders such as encephalomyopathy, nephropathy, and Leigh syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COQ10A antibody 29496-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |